GenePage for the esrE gene of Escherichia coli K-12

Primary Gene Name: esrE
EcoGene Accession Number: EG31191
K-12 Gene Accession Number: ECK4519
MG1655 Gene Identifier: b4707
Gene Name Mnemonic: Essential sRNA in E. coli
Alternate Gene Symbols: None
Description: putative sRNA of unknown function
  # bp Upstream # bp Downstream
MW: 81732.05 ---------252 nt Pre-Run BlastN
Left End: 4019978
Left Intergenic Region

Name: ubiJ_esrE

Length: 252 bp overlap

Orientation: Codirectional+

Left_end: 4019978

Right_end: 4020229

Centisome: 86.61

Genomic Address
Clockwise
Minute or Centisome (%) = 86.61
Right End: 4020229
Right Intergenic Region

Name: esrE_ubiB

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 4020226

Right_end: 4020229

Centisome: 86.61

Earlier results identifying an essential sRNA esrE in the ubiJ(yigP) gene could not be reproduced in Salmonella or E. coli; no Salmonella esrE sRNA was detectable by Northern blots or 5' RACE; the ubiJ(yigP) gene is not essential in E. coli and can be deleted; the ubiJ(yigP) deletion strain forms small colonies aerobically and has a reduced growth yield in liquid medium; ubiJ(yigP) is required for ubiquinone but not menaquinone biosynthesis aerobically, but is not required for anaerobic ubiquinone production; the aerobic growth defects of an E. coli ubiJ(yigP) deletion can be complemented by wildtype ubiJ on a plasmid and by a codon-scrambled version of ubiJ that makes the same UbiJ protein sequence, indicating that the growth defect is due to UbiJ protein synthesis, not the esrE sRNA, in contrast to earlier results; a frameshifted version of ubiJ(yigP) on a plasmid failed to complement the growth defect; the C-terminal 50 codons of ubiJ(yigP) are sufficient for complementation of the growth defect and ubiquinone synthesis defect associated with aerobic growth of the ubiJ(yigP) deletion strain; the biochemical functional of UbiJ is unknown but the authors propose that UbiJ could serve as a carrier of the isoprenoid hydrophobic tail prior to the action of monooxygenases and methyltransferases (Aussel, 2013). The 5' and 3' ends of a putative esrE sRNA were mapped within the ubiJ(yigP) gene; the authors reported that the growth defect associated with a (ubiJ)yigP deletion could be complemented with a minimal esrE gene; the authors reported frameshifts do not disrupt the ability of an esrE clone to complement, indicating an RNA not protein product is responsible for the complementation, but this result was later refuted; the 3' end of esrE corresponds to the stop codon of yigP; there is no obvious Rho-independent terminator at the end of esrE (Chen, 2012).

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII