GenePage for the ghoT gene of Escherichia coli K-12

Primary Gene Name: ghoT
EcoGene Accession Number: EG14342
K-12 Gene Accession Number: ECK4122
MG1655 Gene Identifier: b4559
Gene Name Mnemonic: Ghost cells
Alternate Gene Symbols: yjdO
Description: Toxin of GhoTS toxin-antitoxin pair; membrane-lytic protein; stimulator of persister cell formation
  # bp Upstream # bp Downstream
MW: 6555.06 ---------57 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4352908
Left Intergenic Region

Name: ghoS_ghoT

Length: 27 bp gap

Orientation: Codirectional+

Left_end: 4352881

Right_end: 4352907

Centisome: 93.78

Genomic Address
Clockwise
Minute or Centisome (%) = 93.78
Right End: 4353081
Right Intergenic Region

Name: ghoT_lysU

Length: 118 bp gap

Orientation: Convergent

Left_end: 4353082

Right_end: 4353199

Centisome: 93.78

The GhoT(YjdO) toxin is a membrane-lytic protein that produces 60% non-viable ghost cells when overproduced and increases the formation of persister cells among the survivors; the GhoST toxin-antitoxin pair is proposed to define a new class of TA pairs, named type V, because the GhoS(YjdK) antitoxin is an RNase that cleaves the toxin mRNA; the NMR structure of GhoS (PDB:2LLZ) resembles the Cas2 RNase of the CRISPR system (Wang, 2012; Van Melderen, 2012). Toxin endoribonuclease MqsR degrades ghoS antitoxin mRNA but not ghoT toxin mRNA, amplifying the GhoT toxin production as a stress response (Wang, 2013). MqsR induction increases GhoT production; GhoT is localized to poles and does not cause cell lysis during normal physiology but does impair membrane function, causing a reduction of cellular ATP and PMF energy sources, a lowered metabolism, and an increase in the number of persistent (dormant) cells formed in the presence of antibiotics (Cheng, 2013).

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BamHI EcoRI HindIII