GenePage for the gnsB gene of Escherichia coli K-12

Primary Gene Name: gnsB
EcoGene Accession Number: EG13837
K-12 Gene Accession Number: ECK1544
MG1655 Gene Identifier: b1550
Gene Name Mnemonic: secG Null mutant Suppresor
Alternate Gene Symbols: ydfY
Description: Multicopy suppressor of secG(Cs) and fabA6(Ts), Qin prophage; overexpression increases unsaturated fatty acid content of phospholipids; gnsA paralog
  # bp Upstream # bp Downstream
MW: 6547.67 ---------57 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 1637609
Left Intergenic Region

Name: ydfO_gnsB

Length: 151 bp gap

Orientation: Convergent

Left_end: 1637458

Right_end: 1637608

Centisome: 35.28

Genomic Address
Counterclockwise
Minute or Centisome (%) = 35.28
Right End: 1637782
Right Intergenic Region

Name: gnsB_ynfN

Length: 171 bp gap

Orientation: Codirectional-

Left_end: 1637783

Right_end: 1637953

Centisome: 35.28

The Escherichia coli CFT073 gnsB ortholog is annotated as an 88 amino acid protein due to extension of N-terminus. However, homology does not extend into upstream region and K-12 has an in-frame stop in N-terminal extension region. Therefore GnsB of CFT073 is probably also 57 aa long. Conservation with GnsA paralog and presence of excellent gnsB RBS also supports this conclusion. PRediction of start codon indicated is to allow more spacing between the ATG and the RBS.

Go to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePageGo to GenePage

BamHI EcoRI HindIII