GenePage for the ecpE gene of Escherichia coli K-12

Primary Gene Name: ecpE
EcoGene Accession Number: EG13561
K-12 Gene Accession Number: ECK0288
MG1655 Gene Identifier: b0289
Gene Name Mnemonic: E. coli common pilus
Alternate Gene Symbols: matF; yagV
Description: Periplasmic pilus chaperone required for ECP formation
  # bp Upstream # bp Downstream
MW: 26628.30 ---------236 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 304495
Left Intergenic Region

Name: ykgJ_ecpE

Length: 312 bp gap

Orientation: Codirectional-

Left_end: 304183

Right_end: 304494

Centisome: 6.55

Genomic Address
Counterclockwise
Minute or Centisome (%) = 6.56
Right End: 305205
Right Intergenic Region

Name: ecpE_ecpD

Length: 32 bp overlap

Orientation: Codirectional-

Left_end: 305174

Right_end: 305205

Centisome: 6.57

The E. coli O18ac:K1:H7 NMEC ecpABCDE(matBCDEF) genes are all individually required for E. coli common pilus (ECP) formation in the pathogenic E.coli NMEC strains and ECP production enhances low-temperature biofilm formation in NMEC and and also in K-12 when the silent ecp operon is expressed (Pouttu, 2001; Lehti, 2010; Garnett, 2012). The first 27 aa of EcpE are predicted to be a type I signal peptide. Pfam PF00345 member. ecpB and ecpE are paralogs.

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BamHI EcoRI HindIII