GenePage for the arfA gene of Escherichia coli K-12

Primary Gene Name: arfA
EcoGene Accession Number: EG11968
K-12 Gene Accession Number: ECK3278
MG1655 Gene Identifier: b4550
Gene Name Mnemonic: Alternative ribosome-rescue factor
Alternate Gene Symbols: yhdL
Description: Alternative stalled-ribosome rescue factor A; binds 50S ribosomal subunits, synthetic lethal with ssrA; recruits RF-2
  # bp Upstream # bp Downstream
MW: 8171.38 ---------72 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3438431
Left Intergenic Region

Name: mscL_arfA

Length: 4 bp overlap

Orientation: Convergent

Left_end: 3438431

Right_end: 3438434

Centisome: 74.08

Genomic Address
Counterclockwise
Minute or Centisome (%) = 74.08
Right End: 3438649
Right Intergenic Region

Name: arfA_zntR

Length: 55 bp gap

Orientation: Codirectional-

Left_end: 3438650

Right_end: 3438704

Centisome: 74.08

ArfA recruits release factor RF-2 to release tRNA from stalled ribosomes (Shimizu, 2012; Chadani, 2012). ArfA can rescue stalled ribosomes at the end of a non-stop mRNA without trans-translation (Chadani, 2010). arfB(yaeJ) is a multicopy suppressor of the synthetic lethality of ssrA and arfA (Chadani, 2011a). ArfA translation is regulated by SsrA: in the absence of SsrA a 55 aa truncated ArfA missing the last 18 aa is released from the ribosome and is functional in ribosome rescue (Chadani, 2011b; Garza-Sánchez, 2011). DUF331 family.

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BamHI EcoRI HindIII