GenePage for the gadE gene of Escherichia coli K-12

Primary Gene Name: gadE
EcoGene Accession Number: EG11544
K-12 Gene Accession Number: ECK3496
MG1655 Gene Identifier: b3512
Gene Name Mnemonic: Glutamic acid decarboxylase
Alternate Gene Symbols: yhiE; yhiT
Description: Transcriptional regulator of gadA and gadBC
  # bp Upstream # bp Downstream
MW: 20599.39 ---------175 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3658366
Left Intergenic Region

Name: arrS_gadE

Length: 311 bp gap

Orientation: Divergent

Left_end: 3658055

Right_end: 3658365

Centisome: 78.81

Genomic Address
Clockwise
Minute or Centisome (%) = 78.82
Right End: 3658893
Right Intergenic Region

Name: gadE_mdtE

Length: 338 bp gap

Orientation: Codirectional+

Left_end: 3658894

Right_end: 3659231

Centisome: 78.83

The 750 bp region upstream of gadE is termed the Sensory Integration Region and contains sites needed for the regulation of three promoters (P1, -124; P2, -324/317; P3, -566) by activators YdeO, GadX, an a proposed unknown MnmE-dependent regulator, as well as controlling activation by GadE, GadW and EvgA; no Crp regulation or shorter transcripts were observed (Sayed, 2009). The ArrS sRNA is encoded on the complementary strand of the gadE 5' UTR and is GadE-regulated (Aiso, 2011). The presence of a novel putative sRNA ending at -750 was noted as data not shown (Sayed, 2009). GadE overexpression confers acid resistance. GadE is induced by YdeO overexpression. GadE is in the EvgAS regulon. GadE is a LuxR family regulator. GadE is possibly involved in the regulation of type III secretion systems in enterohemorrhagic E. coli O157:H7. Required for stationary phase-induced, pH 5.5 growth medium-induced, and EvgA-induced acid resistance. GadE and phosphorylated RcsB form a protein complex to regulate glutamate-, arginine- and lysine-dependent acid resistance (Krin, 2010).

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BamHI EcoRI HindIII