GenePage for the acuI gene of Escherichia coli K-12

Primary Gene Name: acuI
EcoGene Accession Number: EG11315
K-12 Gene Accession Number: ECK3241
MG1655 Gene Identifier: b3253
Gene Name Mnemonic: Acrylate utilization
Alternate Gene Symbols: yhdH
Description: Putative acryloyl-CoA reductase
  # bp Upstream # bp Downstream
MW: 34723.76 ---------324 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3403484
Left Intergenic Region

Name: csrD_acuI

Length: 151 bp gap

Orientation: Divergent

Left_end: 3403333

Right_end: 3403483

Centisome: 73.32

Genomic Address
Clockwise
Minute or Centisome (%) = 73.32
Right End: 3404458
Right Intergenic Region

Name: acuI_yrdF

Length: 36 bp gap

Orientation: Codirectional+

Left_end: 3404459

Right_end: 3404494

Centisome: 73.35

acuI(yhdH) mutants are hypersensitive to acrylate (100-fold) and 3-hydroxypropionate (10-fold); AcuI(YdhH) is proposed to have a role in endogenous and/or exogenous acrylate detoxification (Todd, 2012). The acuI gene of R. sphaeroides has been shown to be an acryloyl-CoA reductase yielding propionyl-CoA (Schneider, 2012). A mutation in the acuI gene of R. sphaeroides confers hypersensitivity to acrylate and results in a reduced rate of acrylate metabolism (Sullivan, 2011). E. coli acuI(yhdH), R. sphaeroides acuI, and six other MDR012 genes are all able to complement the acrylate hypersensitivity of an E. coli acuI(yhdH) mutant, indicating their functional equivalence (Todd, 2012). AcuI(YdhH) is a member of the medium chain reductase/dehydrogenase (MDR) family, MDR012 sub-family. 3/148 AcuI(YhdH) peptide masses from MS analysis confirm this start codon and indicate that the initial Met is not removed (Maillet, 2007).

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BamHI EcoRI HindIII