GenePage for the gspB gene of Escherichia coli K-12

Primary Gene Name: gspB
EcoGene Accession Number: EG11263
K-12 Gene Accession Number: ECK3309
MG1655 Gene Identifier: b3322
Gene Name Mnemonic: General secretory pathway
Alternate Gene Symbols: pinO; pioO
Description: Part of H-NS-silenced gsp-alpha (T2SS-alpha) divergon, type II protein secretion; cloned gsp-alpha (T2SS-alpha) divergon secretes ChiA, requires gspAB operon
  # bp Upstream # bp Downstream
MW: 15876.00 ---------139 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3453508
Left Intergenic Region

Name: rpsJ_gspB

Length: 237 bp gap

Orientation: Codirectional-

Left_end: 3453271

Right_end: 3453507

Centisome: 74.40

Genomic Address
Counterclockwise
Minute or Centisome (%) = 74.40
Right End: 3453927
Right Intergenic Region

Name: gspB_gspA

Length: 1 bp gap

Orientation: Codirectional-

Left_end: 3453928

Right_end: 3453928

Centisome: 74.41

GspB is a distant homolog or convergent analog of exeB of A. hydrophila, which is required for protein export, specifically for the export and assembly of secretin (ExeD/GspD) into the outer membrane. GspB has a single predicted N-terminal transmembrane segment (28-50), followed by a predicted periplasmic domain, which may interact with GspA. GspA may interact with GspB. A divergon is a regulon comprised of two adjacent divergent operons or genes. gspB was initially identified as an ORF that might be the 11.3 kDa PinO protein synthesized in the absence of isoleucine in relA stains (Guzman, 1991). But GspB is predicted to be 15.9 kDa and has isoleucine residues and there is no evidence that gspB encodes the PinO peptide or that it binds calcium. No homology to calmodulin or other calcium-binding proteins is predicted. gspB may have been mistaken for pinO due to use of a downstream ATG as start codon, but the 11 kD C-terminal fragment does have an Ile reside in it, making gspB inconsistent with pinO. pioO was the prior gene name assigned to pinO since the gene name pin was already taken.

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BamHI EcoRI HindIII