GenePage for the damX gene of Escherichia coli K-12

Primary Gene Name: damX
EcoGene Accession Number: EG11183
K-12 Gene Accession Number: ECK3375
MG1655 Gene Identifier: b3388
Gene Name Mnemonic: DNA adenine methylase
Alternate Gene Symbols: yhfB
Description: Cell division protein, binds septal ring; bile salts resistance
  # bp Upstream # bp Downstream
MW: 46161.91 ---------428 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3516020
Left Intergenic Region

Name: dam_damX

Length: 106 bp gap

Orientation: Codirectional-

Left_end: 3515914

Right_end: 3516019

Centisome: 75.75

Genomic Address
Counterclockwise
Minute or Centisome (%) = 75.75
Right End: 3517306
Right Intergenic Region

Name: damX_aroB

Length: 91 bp gap

Orientation: Codirectional-

Left_end: 3517307

Right_end: 3517397

Centisome: 75.78

A damX mutant is bile-sensitive, as is a downstream dam mutant, but the damX phenotype is complemented by a damX clone indicating it is not a polar effect on dam (Arends, 2010). Overexpression of DamX induces cell filamentation (Lyngstadaas, 1995). Overexpression of DamX causes filamentous biofilm formation (Tenorio, 2003). DamX has a 100 aa cytoplasmic domain, a single transmembrane region, and a C-terminal SPOR domain. DamX localizes to the septal ring, via the SPOR domain, and damX mutants are defective in cell division (Gerding, 2009; Arends, 2010). DamX interacts with FtsQ and other cell division proteins in a bacterial two-hybrid assay and may be a negative regulator of FtsQ function (Arends, 2010). HT_Cmplx49_Mem: DamX+BamA.

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BamHI EcoRI HindIII