GenePage for the hisF gene of Escherichia coli K-12

Primary Gene Name: hisF
EcoGene Accession Number: EG10448
K-12 Gene Accession Number: ECK2020
MG1655 Gene Identifier: b2025
Gene Name Mnemonic: Histidine
Alternate Gene Symbols: None
Description: Imidazole glycerol phosphate (IGP) synthase, cyclase subunit
  # bp Upstream # bp Downstream
MW: 28454.45 ---------258 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2095844
Left Intergenic Region

Name: hisA_hisF

Length: 19 bp overlap

Orientation: Codirectional+

Left_end: 2095844

Right_end: 2095862

Centisome: 45.15

Genomic Address
Clockwise
Minute or Centisome (%) = 45.15
Right End: 2096620
Right Intergenic Region

Name: hisF_hisI

Length: 7 bp overlap

Orientation: Codirectional+

Left_end: 2096614

Right_end: 2096620

Centisome: 45.17

The N-terminus of purified HisF was sequenced and the fMet is not clipped, but HisF is omitted from the Verified Set because its natural RBS was not used; the natural RBS is very good and this is almost certainly the in vivo start (Klem, 1993).

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BamHI EcoRI HindIII