GenePage for the entF gene of Escherichia coli K-12

Primary Gene Name: entF
EcoGene Accession Number: EG10264
K-12 Gene Accession Number: ECK0579
MG1655 Gene Identifier: b0586
Gene Name Mnemonic: Enterochelin
Alternate Gene Symbols: None
Description: Enterochelin synthase, component F; multifunctional: holo-EntF(PCP), primed by EntD; seryl-AMP, seryl-S-EntF synthase; DHB-Ser-S-EntF intermediate formation; enterobactin biosynthesis
  # bp Upstream # bp Downstream
Verified Start MW: 141990.95 ---------1293 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 614157
Left Intergenic Region

Name: ybdZ_entF

Length: 4 bp overlap

Orientation: Codirectional+

Left_end: 614157

Right_end: 614160

Centisome: 13.23

Genomic Address
Clockwise
Minute or Centisome (%) = 13.23
Right End: 618038
Right Intergenic Region

Name: entF_fepE

Length: 215 bp gap

Orientation: Codirectional+

Left_end: 618039

Right_end: 618253

Centisome: 13.32

EntF is a four domain (condensation-adenylation-peptidyl carrier protein-thioesterase) nonribosomal peptide synthase responsible for synthesis of the siderophore enterobactin (Shaw-Reid, 1999). The chain-releasing thioesterase (TE) domain of EntF is both a cyclotrimerizing lactone synthase and an elongation catalyst for ester-bond formation between covalently tethered dihydroxybenzoate-serine moieties (Shaw-Reid, 1999). The adenylation activity of EntF is stimulated by the co-purifying MbtH-like factor YbdZ; the affinity of EntF for L-ser was improved 15-fold in the presence of YbdZ (Felnagle, 2010). In addition to its role in iron acquisition, enterobactin plays a role in oxidative stress protection and is required to form colonies on M9 minimal media aerobically (Adler, 2014).

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BamHI EcoRI HindIII