GenePage for the dapE gene of Escherichia coli K-12

Primary Gene Name: dapE
EcoGene Accession Number: EG10208
K-12 Gene Accession Number: ECK2467
MG1655 Gene Identifier: b2472
Gene Name Mnemonic: DiAminoPimelate
Alternate Gene Symbols: msgB
Description: N-succinyl-diaminopimelate desuccinylase, DAP/lysine biosynthesis, contains Zn(2+)/Co(2+)
  # bp Upstream # bp Downstream
MW: 41269.01 ---------375 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 2591607
Left Intergenic Region

Name: yffB_dapE

Length: 3 bp gap

Orientation: Codirectional+

Left_end: 2591604

Right_end: 2591606

Centisome: 55.83

Genomic Address
Clockwise
Minute or Centisome (%) = 55.83
Right End: 2592734
Right Intergenic Region

Name: dapE_ypfN

Length: 27 bp gap

Orientation: Codirectional+

Left_end: 2592735

Right_end: 2592761

Centisome: 55.86

A dapE mutant is not in the Keio collection; DAP auxotrophs require added DAP to grow and since no DAP was added during mutant construction, no auxotrophic mutants were recovered. dapE(msgB) is a multicopy suppressor of grpE(Ts). Salmonella DapE also has Zn(2+)/Mn(2+)-dependent Asp-X dipeptidase activity. 7/81 DapE peptide masses from MS analysis confirm this start codon and indicate that the initial Met is removed (Maillet, 2007).

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BamHI EcoRI HindIII