GenePage for the btuB gene of Escherichia coli K-12

Primary Gene Name: btuB
EcoGene Accession Number: EG10126
K-12 Gene Accession Number: ECK3958
MG1655 Gene Identifier: b3966
Gene Name Mnemonic: B12 uptake
Alternate Gene Symbols: bfe; cer; dcrC
Description: Vitamin B12 outer membrane receptor; receptor for E colicins, phages BF23 and C1
  # bp Upstream # bp Downstream
Verified Start MW: 68407.15 ---------614 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 4163639
Left Intergenic Region

Name: trmA_btuB

Length: 368 bp gap

Orientation: Divergent

Left_end: 4163271

Right_end: 4163638

Centisome: 89.69

Genomic Address
Clockwise
Minute or Centisome (%) = 89.70
Right End: 4165483
Right Intergenic Region

Name: btuB_murI

Length: 56 bp overlap

Orientation: Codirectional+

Left_end: 4165428

Right_end: 4165483

Centisome: 89.74

BtuB translation is regulated by adenosylcobalamin binding directly to btuB mRNA which contains a vitamin B12-responsive riboswitch (Nou, 2000; Nahvi, 2002; Johnson, 2012). The btuB mutants in the Keio collection have been shown to harbor an intact copy of btuB in addition to the deletion allele (Yamamoto, 2009). btuB is a non-essential gene (Koster, 1991). btuB deletions in the Keio collection also disable the 5' end of the downstream essential murI gene due to an unusually large 56 bp gene overlap, explaining why the btuB Keio mutants are merodiploid (Aufrère, 1986; Zhou, 2012).

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BamHI EcoRI HindIII