GenePage for the bioH gene of Escherichia coli K-12

Primary Gene Name: bioH
EcoGene Accession Number: EG10122
K-12 Gene Accession Number: ECK3399
MG1655 Gene Identifier: b3412
Gene Name Mnemonic: Biotin
Alternate Gene Symbols: bioB
Description: Pimeloyl-ACP methyl ester carboxylesterase; short acyl chain carboxylesterase, binds CoA; biotin synthesis blocked prior to pimeloyl CoA formation
  # bp Upstream # bp Downstream
MW: 28505.01 ---------256 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3544074
Left Intergenic Region

Name: yhgA_bioH

Length: 28 bp gap

Orientation: Convergent

Left_end: 3544046

Right_end: 3544073

Centisome: 76.35

Genomic Address
Counterclockwise
Minute or Centisome (%) = 76.35
Right End: 3544844
Right Intergenic Region

Name: bioH_gntX

Length: 37 bp gap

Orientation: Divergent

Left_end: 3544845

Right_end: 3544881

Centisome: 76.37

BioC and BioH are required to synthesize pimeloyl-ACP, a biotin precursor, utilizing the enzymes of fatty acid synthesis to add acyl groups to malonyl-CoA in two cycles to form pimeloyl-ACP methyl ester, which is hydrolyzed by BioH (Lin, 2010). S. marcescens bioH complements E. coli bioH. The N-terminal methionine is not removed, as indicated by the intact mass of the overexpressed protein.

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BamHI EcoRI HindIII