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EcogeneTopic Page
TopicPage for Keio Collection of Escherichia coli K-12
 | Essential Genes |
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Description:
Keio Collection
Details:
The Keio collection of 3985 E. coli K-12 BW25113 deletion/insertion mutations (Baba, 2006) is being distributed either in its entirety from Japan or as individual strains from the Coli Genetic Stock Center. These strains have individual kan cassette replacements of non-essential genes flanked by FRT sites allowing excision using the FLP recombinase on plasmid pCP20.
The subTopics of this Topic include a series of published results of high throughput phenotypic screens using the Keio collection. This allows the easy retrieval and review of the various Keio mutant phenotypes reported.
We also include as subTopic the Keio strains that have been individually verified and/or characterized in various laboratories who have received the collection.
Additional information about individual mutants in the Keio collection and individual ASKA clones are available by clicking a blue ASKA/KEIO button on the GenePages.
- 2008
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Typas A, Nichols RJ, Siegele DA, Shales M, Collins SR, Lim B, Braberg H, Yamamoto N, Takeuchi R, Wanner BL, Mori H, Weissman JS, Krogan NJ, Gross CA (2008) High-throughput, quantitative analyses of genetic interactions in E. coli. Nat Methods 5:781-7
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Silhavy TJ, Gitai Z (2008) Sex to the rescue. Nat Methods 5:759-60
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Butland G, Babu M, Díaz-Mejía JJ, Bohdana F, Phanse S, Gold B, Yang W, Li J, Gagarinova AG, Pogoutse O, Mori H, Wanner BL, Lo H, Wasniewski J, Christopolous C, Ali M, Venn P, Safavi-Naini A, Sourour N, Caron S, Choi JY, Laigle L, Nazarians-Armavil A, Deshpande A, Joe S, Datsenko KA, Yamamoto N, Andrews BJ, Boone C, Ding H, Sheikh B, Moreno-Hagelseib G, Greenblatt JF, Emili A (2008) eSGA: E. coli synthetic genetic array analysis. Nat Methods 5:789-95
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Typas A, Nichols RJ, Siegele DA, Shales M, Collins SR, Lim B, Braberg H, Yamamoto N, Takeuchi R, Wanner BL, Mori H, Weissman JS, Krogan NJ, Gross CA (2008) High-throughput, quantitative analyses of genetic interactions in E. coli. Nat Methods 5:781-787
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Tamae C, Liu A, Kim K, Sitz D, Hong J, Becket E, Bui A, Solaimani P, Tran KP, Yang H, Miller JH (2008) Determination of antibiotic hypersensitivity among 4,000 single-gene-knockout mutants of Escherichia coli. J Bacteriol 190:5981-8
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Plaimas K, Mallm JP, Oswald M, Svara F, Sourjik V, Eils R, König R (2008) Machine learning based analyses on metabolic networks supports high-throughput knockout screens. BMC Syst Biol 2:67
- 2007
- 2006
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Baba T, Ara T, Hasegawa M, Takai Y, Okumura Y, Baba M, Datsenko KA, Tomita M, Wanner BL, Mori H (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol Syst Biol 2:2006.0008
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