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Showing 11-20 of 309 paper
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Ling,2010ExperimentsMotility and FlagellaE. coli K-12 W3110Agilent E. coli MicroarraysEffects of luxS-deletion on bacterial phenotypic traits.Matthew Wook Chang
Horinouchi,2010Experimentsethanol stress; adaptive evolution E. coli K-12 W3110 custom-designed microarray of E. coli W3110/ Affymetrix platform A whole-transcriptome analysis to analyze adaptive evolution due to ethanol stress was completed.Hiroshi Shimizu
Fantino,2010Experimentscobalt stress E. coli K-12 MC4100 Gif/Orsay DNA microarrays PlatformAnalysis used RNA extracted from Escherichia coli exposed or not to cobalt. Experiments were done with E. coli MC4100 cultures exposed to CoCl2 (250 µM) in mid-exponential phase during 30 min. Comparisons were made across multiple arrays, using E. coli MC4100 DNA microarrays spotted with PCR products at the Gif/Orsay DNA microarrays Platform.Frédéric Barras
Portnoy,2010ExperimentsAerobic-Anaerobic Shift E. coli K-12 MG1655 vs. ECOM4LA Affymetrix GeneChip E. coli Genome 2.0 arrays The data represents a genome-wide transcriptome studies of wild-type and ECOM4LA (Escherichia coli cytochrome oxidase mutant 4 (del:cydAB, cyoABCD, cbdAB, and ygiN)) cells under aerobic and anaerobic conditions. Bacterial strains were cultured at 37 degrees C in M9 medium (4 g/L glucose) to OD600=0.6 for the WT and 0.25 for ECOM4LA.Bernhard �. Palsson
King,2010Experimentsacid tolerance responsesE. coli K-12 MG1655; E. coli O157:H7 SakaiAffymetrix GeneChip E. coli Genome 2.0 arrays Thea King
Singh,2010ExperimentsIncreased mutation frequency; redox-impaired Escherichia E. coli K-12 BW25113 Affymetrix E. coli Antisense Genome Array Whole-genome transcriptional profiles were compared between wild-type cells and a double deletion mutant to isolates genes involved in DNA repair. Ryan T. Gil
McLean,2010ExperimentsPeroxynitrite toxicity; Oxidative stressE. coli K-12 (MG1655)MWG E. coli K12 ArrayPeroxynitrite was added directly to growing cultures in log phase which triggered an up-regulation of oxidative stress response genes. Transcriptomic analysis of cells treated with hydrogen peroxide alone also revealed up-regulation of oxidative stress response genes but not of many other genes that are up-regulated by peroxynitrite. The authors show that the cellular responses to peroxynitrite and hydrogen peroxide are distinct.Samantha McLean
Cariss,2010ExperimentsCreBC-Dependent Colicin E2 Tolerance; YieJ E. coli K-12 MG1655 Slides; Operon Array-Ready E. coli set 1.0 The data represents CreB-dependent gene upregulation in the CreC hyperactive mutant CTX6.Matthew B. Avison
Habdas,2010ExperimentsMotility E. coli K-12 MG1655 Affymetrix E. coli Genome 2.0 Array An E. coli K-12 qseD mutant was also constructed in order to compare and contrast the effects of the HTH truncation in EHEC on genetic regulation.Vanessa Sperandi
Shepherd,2010Experimentscellular respirationE. coli K-12 (BW25113) vs. isogenic KEIO cydBSlides; Ocimum Biosolution E. coli K12 arraysThe study demonstrates that the loss of cydB causes diminished respiration rates in E. coli K-12, despite the maintenance of a wild-type growth rate in the mutant. The wild type strain exhibited a higher rate of respiration as compared with the cydB strain, and a paired t-test confirmed this observation with 99.99% confidence. Mark Shepherd