GenePage for the rhsA gene of Escherichia coli K-12

Primary Gene Name: rhsA
EcoGene Accession Number: EG10846
K-12 Gene Accession Number: ECK3582
MG1655 Gene Identifier: b3593
Gene Name Mnemonic: Recombination hotspot
Alternate Gene Symbols: None
Description: Rhs protein with putative toxin 55 domain; putative polysaccharide synthesis/export protein; putative neighboring cell growth inhibitor
  # bp Upstream # bp Downstream
MW: 156320.78 ---------1377 aa Pre-Run BlastP UniProt
Pre-Run BlastP NR+Env
Left End: 3762183
Left Intergenic Region

Name: yibF_rhsA

Length: 227 bp gap

Orientation: Divergent

Left_end: 3761956

Right_end: 3762182

Centisome: 81.05

Genomic Address
Clockwise
Minute or Centisome (%) = 81.05
Right End: 3766316
Right Intergenic Region

Name: rhsA_yibA

Length: 20 bp gap

Orientation: Codirectional+

Left_end: 3766317

Right_end: 3766336

Centisome: 81.14

It has been suggested that all Rhs systems are involved in intercellular growth inhibition, suggesting that the Rhs proteins are secreted from E. coli and that the variable C-terminal domains are toxic to neighboring cells when secreted to the outside of the cell (Koskiniemi, 2013). RhsA could be cross-linked to Kps proteins in a K5+ K-12 strain; an rhsA mutant has reduced K5 polysaccharide biosynthesis in K-12 K5+ strain (McNulty, 2006). RhsA has 28 core RHS repeats including several repeats with YD dipeptides (Feulner, 1990). RhsA is a predicted polymorphic toxin that has a C-terminal conserved Toxin 55 domain that is a predicted RNase toxin (Pfam PF15606) that might be an intercellular growth inhibition module in that it also occurs in secreted bacterial insecticidal toxins and intercellular signaling proteins; the downstream yibA gene is predicted to encode an immunity gene (Zhang, 2012).

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BamHI EcoRI HindIII